| Autor: |
Niu BL; Sericultural Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China., Meng ZQ, Tao YZ, Lu SL, Weng HB, He LH, Shen WF |
| Jazyk: |
angličtina |
| Zdroj: |
Acta biochimica et biophysica Sinica [Acta Biochim Biophys Sin (Shanghai)] 2005 Nov; Vol. 37 (11), pp. 728-36. |
| DOI: |
10.1111/j.1745-7270.2005.00106.x |
| Abstrakt: |
We have identified Bombyx mori transformer-2 gene (Bmtra-2) cDNA by blasting the EST database of B. mori. It was expressed in the whole life of the male and female silkworm and was observed as a band of 1.3 kb by Northern blot analysis. By comparing corresponding ESTs to the Bmtra-2 DNA sequence, it was revealed that there were eight exons and seven introns, and all splice sites of exons/introns conformed to the GT/AG rule. Bmtra-2 pre-mRNA can produce multiple mRNAs encoding six distinct isoforms of BmTRA-2 protein using an alternative splicing pathway during processing. Six types of Bmtra-2 cDNA clones were identified by reverse transcription-polymerase chain reaction. All isoforms of BmTRA-2 protein contain two arginine/serine-rich domains and one RNA recognition motif, showing striking organizational similarity to Drosophila TRA-2 proteins. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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