| Autor: |
Grönroos JO; Department of Pathology, University of Turku, Finland. juha.gronroos@paimio.fi, Salonen JH, Viander M, Nevalainen TJ, Laine VJ |
| Jazyk: |
angličtina |
| Zdroj: |
Scandinavian journal of immunology [Scand J Immunol] 2005 Oct; Vol. 62 (4), pp. 413-9. |
| DOI: |
10.1111/j.1365-3083.2005.01678.x |
| Abstrakt: |
The complement system is regarded as an important component of the innate defence system against invading bacteria. However, synergistic actions between the complement and the other components of innate immunity are incompletely known. Human group IIA phospholipase A(2) (hGIIA PLA(2)) is an effective antibacterial enzyme in serum of patients with severe bacterial infections. Our aim was to investigate the significance of complement and hGIIA PLA(2) in acute phase serum. Serum samples were collected from patients with acute bacterial infections and from healthy control subjects. We prepared hGIIA PLA(2)-depleted serum by immunoadsorption and inhibited the activity of complement by a specific inhibitor, compstatin. The bactericidal effects of treated and untreated serum were compared by incubating Staphylococcus aureus and Listeria monocytogenes in the presence of serum. Acute phase serum effectively killed S. aureus and L. monocytogenes, and depletion of hGIIA PLA(2) significantly reduced the antibacterial effect. Complement had a weak bactericidal effect against L. monocytogenes. We conclude that hGIIA PLA(2) is the major antibacterial factor in human acute phase serum against the gram-positive bacteria S. aureus and L. monocytogenes, exceeding complement in efficiency. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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