| Autor: |
Verma DD; Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA., Levchenko TS, Bernstein EA, Torchilin VP |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of controlled release : official journal of the Controlled Release Society [J Control Release] 2005 Nov 28; Vol. 108 (2-3), pp. 460-71. Date of Electronic Publication: 2005 Oct 17. |
| DOI: |
10.1016/j.jconrel.2005.08.029 |
| Abstrakt: |
ATP-loaded liposomes (ATP-L) infused into Langendorff-instrumented isolated rat hearts protect the mechanical functions of the myocardium during ischemia/reperfusion. The left ventricular developed pressure (LVDP) at the end of the reperfusion in the ATP-L group recovered to 72% of the baseline (preservation of the systolic function) compared to 26%, 40%, and 51% in the groups treated with Krebs-Henseleit (KH) buffer, empty liposomes (EL), and free ATP (F-ATP), respectively. The ATP-L-treated group also showed a significantly lower left ventricular end diastolic pressure (LVEDP; better preservation of the diastolic function) after ischemia/reperfusion than controls. After incubating the F-ATP and ATP-L with ATPase, the protective effect of the F-ATP was completely eliminated because of ATP degradation, while the protective effect of the ATP-L remained unchanged. Fluorescence microscopy confirmed the accumulation of liposomes in ischemic areas, and the net ATP in the ischemic heart increased with ATP-L. Our results suggest that ATP-L can effectively protect myocardium from ischemic/reperfusion damage. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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