| Autor: |
Ruzgas TA; Department of Enzyme Chemistry, Institute of Biochemistry, Lithuanian Academy of Sciences., Razumas VJ, Kulys JJ |
| Jazyk: |
angličtina |
| Zdroj: |
Biosensors & bioelectronics [Biosens Bioelectron] 1992; Vol. 7 (4), pp. 305-8. |
| DOI: |
10.1016/0956-5663(92)87009-e |
| Abstrakt: |
Ellipsometric immunosensors for gamma-interferon (gamma-INF) and human serum albumin (HSA) have been developed using gamma-INF and HSA covalently immobilized onto silicon plates. Detection is achieved by measuring the adsorption decrease (gamma, mg m-2) of monoclonal immunoglobulin G against gamma-INF or immunoglobulin fraction of rabbit blood serum against HSA when the concentration of antigen in mixtures of antibody-antigen is increased. The time of analysis is 50 min, the detection limit of gamma-INF is 15 nM (sensitivity -0.01 mg m-2 nM-1) and of HSA 2.5 nM (-0.06 mg m-2 nM-1). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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