[Determination of fumonisin biosynthesis gene and toxicity of Fusarium moniliforme strains isolated in China].

Autor:	Liu XM; National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China., Wang XY, Qiu MF, Li XF
Jazyk:	čínština
Zdroj:	Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] [Zhonghua Yu Fang Yi Xue Za Zhi] 2005 Jul; Vol. 39 (4), pp. 249-52.
Abstrakt:	Objective: To study the relationship between fumonisin biosynthesis gene and toxicity of Fusarium moniliforme isolated in China. Methods: The toxigenic gene of 29 Fusarium moniliforme isolated from different provinces and varied food samples were determined. Eighteen fum5-positive strains were selected for biosyhesizing fumonisin and determined by high performance liquid chromatography (HPLC). Results: Twenty-six isolates were identified as fum5 gene positive strains. And all of these strains produced FB(1), FB(2) and FB(3). The amount of FB(1), FB(2) and FB(3) was ranging from 0.41-140.20 mg/kg, 0.06-14.30 mg/kg to 0.30-58.00 mg/kg, except one strain produced a lower level of FB(1) only. It wight be the first report showing a high level fumonisin-producing strain isolated from the sesame sample and identified in the world. The amount of FB(1), FB(2) and FB(3) produced by the isolate was 128.84 mg/kg, 11.80 mg/kg and 14.88 mg/kg. Conclusions: It should have a close relationship between fumonisins biosynthesis gene and toxicity of Fusarium moniliforme isolated in China. The study demonstrated that strain of Fusarium moniliforme might contaminate the sesame sample and produce a high level of fumonisins.
Databáze:	MEDLINE
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