| Autor: |
Neubecker TA; Miami Valley Laboratories, Procter and Gamble Company, Cincinnati, OH 45239-8707., Miller NS, Asquith TN, Driscoll KE |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography [J Chromatogr] 1992 Feb 14; Vol. 574 (2), pp. 340-3. |
| DOI: |
10.1016/0378-4347(92)80049-v |
| Abstrakt: |
To better define involvement of mast cell derived mediators in the pulmonary response to fibrogenic dusts, a rapid and accurate method was required to analyze samples of bronchoalveolar lavage fluid for histamine. Samples of rat lung lavage were analyzed for histamine via high-performance cation-exchange chromatography coupled with post-column derivatization with o-phthaldialdehyde. The fluorescent derivative could be detected to ca. 1 ng/ml of lavage. Recoveries averaged 94.2% with an average relative standard deviation of +/- 5.3%. There were no correlations between amount or fibrogenicity of inhaled dust and subsequent release of histamine into lavage fluid. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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