[Experimental model of primary cultured metastatic melanoma by fine needle aspiration].

Autor:	Oliveira AF; Laboratório de Cultura de Células, Universidade Federal de São Paulo, Escola Paulista de Medicina (UNIFESP/EPM), São Paulo. drandreafernandes@terra.com.br, Gragnani A, Oliveira Filho RS, Santos ID, França SG, Enokihara MM, Ferreira LM
Jazyk:	portugalština
Zdroj:	Acta cirurgica brasileira [Acta Cir Bras] 2005 Sep-Oct; Vol. 20 (5), pp. 390-3. Date of Electronic Publication: 2005 Sep 05.
DOI:	10.1590/s0102-86502005000500009
Abstrakt:	Purpose: Establish an experimental model of metastatic melanoma primary culture cells from fine-needle aspiration biopsies (FNAB). Methods: Two metastatic melanoma primary culture cells from (FNAB) have been developed from patients who had been submitted to excision of metastatic lesions and they were identified by immunohistochemical analyses using S-100 and MB-45 melanoma markers. Results: The culture diagnostic was confirmed by immunohistochemical. The technique of FNAB offers the advantage of providing a sequential analysis of the same tumor nodules throughout treatment, and is a minimally invasive procedure with almost no associated morbidity. Conclusion: The establishment of metastatic melanoma primary culture from FNAB samples showed a viable technique.
Databáze:	MEDLINE
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