Detection and characterization by high-performance liquid chromatography and mass spectrometry of a goat beta-casein associated with a CSN2 null allele.

T) at the 373rd nucleotide of the 7th exon of the gene, which generates a premature stop codon in position 182.
((c) 2005 John Wiley & Sons, Ltd.) -->
Substance Nomenclature: 0 (Caseins)
0 (Milk Proteins)
Entry Date(s): Date Created: 20050924 Date Completed: 20051115 Latest Revision: 20191210
Update Code: 20231215
DOI: 10.1002/rcm.2143
PMID: 16178051
Autor: Cunsolo V; Dipartimento di Scienze Chimiche, Università degli Studi di Catania, Viale A. Doria, 6, 95125 Catania, Italy., Galliano F, Muccilli V, Saletti R, Marletta D, Bordonaro S, Foti S
Jazyk: angličtina
Zdroj: Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2005; Vol. 19 (20), pp. 2943-9.
DOI: 10.1002/rcm.2143
Abstrakt: The identification and characterization of a truncated goat beta-casein, associated with a null beta-casein allele (CSN2(O')), is reported. The truncated beta-casein predicted at the DNA level (NCBI Acc. No. CAB39313) but never observed at the protein level, here named beta-casein O, was detected as a minor component in a goat milk sample from an autochthonous breed from southern Italy, 'Rossa Mediterranea', by reversed-phase high-performance liquid chromatography/electrospray ionization mass spectrometry (RP-HPLC/ESI-MS). The ESI mass spectrum of the intact beta-casein O determined an M(r) value of 18 780 Da (calculated 18 781.5). Characterization of the amino acid sequence, performed by coupling trypsin digestion with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), RP-HPLC/ESI-MS and tandem mass spectrometry (MS/MS), demonstrated that the amino acid sequence corresponds to the 1-166 sequence of mature beta-casein variant A (Acc. No. P33048), thus confirming that the protein is coded by the null allele CSN2(O'), characterized by a transition (C --> T) at the 373rd nucleotide of the 7th exon of the gene, which generates a premature stop codon in position 182.
((c) 2005 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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