Influence of UV-irradiation on enzymes in mouse ocular lens: in vitro studies.
| Autor: | Jain NK; Department of Zoology, School of Sciences, Gujarat University, Ahmedabad, India., Rawal UM |
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| Jazyk: | angličtina |
| Zdroj: | Indian journal of ophthalmology [Indian J Ophthalmol] 1999 Mar; Vol. 47 (1), pp. 25-9. |
| Abstrakt: | Purpose: In vitro study of the enzymes involved in aerobic, anaerobic and hexose monophosphate shunt in ultraviolet radiation exposed mice lenses. Method: Of the selected enzymes, lactic dehydrogenase (LDH) was representative of anaerobic glucose oxidation, succinic dehydrogenase (SDH) of the aerobic oxidation, and Glucose-6-phosphate dehydrogenase (G-6-PDH) of the Hexose Monophosphate (HMP) shunt. Other enzymes studied were ATPase and glutathione reductase (GR). Results: Experiments with mice lenses in vitro showed that transparent lens became opaque following UV-irradiation at 360 nm. Opacification of the lens was accompanied by a change in enzyme activities for energy metabolism. Conclusion: These changes were progressive in a manner analogous to sequential morphological changes, which would be crucial in maintaining lens transparency. |
| Databáze: | MEDLINE |
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