| Autor: |
Mao L; School of Public Health, Nanjing Medical University, Nanjing 210029, China. maoli@njmu.edu.cn, Jin N, Chen J |
| Jazyk: |
čínština |
| Zdroj: |
Se pu = Chinese journal of chromatography [Se Pu] 2005 May; Vol. 23 (3), pp. 282-4. |
| Abstrakt: |
A method for the determination of quercetin in pulp and peel of apples by reversed-phase high performance liquid chromatography with internal standard was developed. Samples were frozen at -80 degrees C for 24 h, then added 6 mol/L hydrochloride under the protection of antioxidant 2,6-di-tert-butyl-p-cresol. The slurry was hydrolyzed thermostatically at 90 degrees C for 2 h and centrifugated for 10 min. The separation was performed on an ODS column (150 mm x 6.0 mm i.d., 5 microm). Methanol-water (55:45, v/v) (pH adjusted to 3.3 with acetic acid) was used as the mobile phase with a flow rate of 1.0 mL/min. The injection volume was 20 microL. The detection wavelength was 370 nm. The results showed that the quercetin content in pulp and in peel were 3.11-10.78 microg/g and 253.57-744.59 microg/g, respectively. The mean recovery of quercetin in apple pulp was 100.4%. The method is simple, accurate, and reliable. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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