| Autor: |
Canudas N; Laboratorio de Fotoquímica y Fotobiología, Departamento de Química, Universidad Simón Bolívar, Venezuela. ncanudas@usb.ve, Zamora D, Villamizar JE, Fuentes J, Castelli C, Taddei A |
| Jazyk: |
angličtina |
| Zdroj: |
Die Pharmazie [Pharmazie] 2005 Aug; Vol. 60 (8), pp. 604-8. |
| Abstrakt: |
The photobiological properties of 6-methoxy-2-naphthylacetic acid (6-MNAA) were studied using a variety of in vitro phototoxicity assays: photohemolysis, photoperoxidation of linoleic acid, photosensitized degradation of histidine and thymine and the Candida phototoxicity test. 6-MNAA was phototoxic in vitro. 6-MNAA reduced nitro blue tetrazolium (NBT) when irradiated with lambda > or = 300 nm in deoxygenated aqueous buffer solution (pH 7.4). NBT can be reduced by reaction with the excited state of 6-MNAA subject to interference with molecular oxygen. The photohemolysis rate was inhibited by the presence of 1,4-diazabicyclo[2.2.2]octane (DABCO), sodium azide (NaN3) and reduced glutathione (GSH). Photoperoxidation of linoleic acid and photosensitized degradation of histidine and thymine were significantly inhibited by sodium azide and reduced glutathione. 6-MNAA was phototoxic to C. albicans, C. lipolytica and C. tropicalis. A mechanism involving singlet oxygen, radicals, and electron transfer reactions is suggested for the observed phototoxicity. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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