| Autor: |
Van Uden W; Laboratory for Pharmacognosy, University of Groningen, The Netherlands., Homan B, Woerdenbag HJ, Pras N, Malingré TM, Wichers HJ, Harkes M |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of natural products [J Nat Prod] 1992 Jan; Vol. 55 (1), pp. 102-10. |
| DOI: |
10.1021/np50079a015 |
| Abstrakt: |
A method has been developed for the large scale isolation of 5-methoxypodophyllotoxin [1] from a high-producing root culture derived from Linum flavum. A closely related lignan, 5'-demethoxy-5-methoxypodophyllotoxin [2], was also present in the root culture and was the cause of the main isolation difficulties. Essential steps in the isolation procedure are CH2Cl2 and XAD-4 extraction and XAD-8 cc followed by Si gel chromatography, using two different mobile phases. The isolated 5-methoxypodophyllotoxin [1] was very pure (greater than 99%) and possessed the desired stereochemical configuration, namely (-)-5-methoxypodophyllotoxin [1]. The in vitro cytotoxicity of 5-methoxypodophyllotoxin [1] against EAT and HeLa cells was determined and compared with those of podophyllotoxin [3], etoposide (VP-16-213) [4], teniposide (VM-26) [5], and 5-methoxypodophyllotoxin-4-beta-D-glucoside [6]. It appeared that 5-methoxypodophyllotoxin [1] has about the same cytotoxic potency as podophyllotoxin [3]. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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