The immune privilege of the eye: human retinal pigment epithelial cells selectively modulate T-cell activation in vitro.

Autor: Kaestel CG; Department of Medical Anatomy, The Panum Institute, University of Copenhagen, Copenhagen, Denmark., Lovato P, Ødum N, Nissen MH, Röpke C
Jazyk: angličtina
Zdroj: Current eye research [Curr Eye Res] 2005 May; Vol. 30 (5), pp. 375-83.
DOI: 10.1080/02713680590934120
Abstrakt: Purpose: To examine the effect of human retinal pigment epithelial (RPE) cells on phytohemagglutinin (PHA) activation of T cells.
Methods: Resting peripheral blood lymphocytes (PBLs) were stimulated with PHA with or without the presence of gamma-irradiated RPE cells. Proliferation and the cell cycle profile were thereafter investigated by 3H-thymidine incorporation and flow cytometric analysis. In addition, the PBLs expression of CD69, major histocompatibility complex (MHC) class I and II, CD3, as well as the IL-2 receptor chains were evaluated by flow cytometry, and the content of IL-2 in cell culture supernatant was measured by ELISA.
Results: Human RPE cells were found to suppress PHA-induced proliferation, cyclin A, IL-2R-alpha and -gamma, and CD71 expression and decrease the production of IL-2; but RPE cells do not inhibit the PHA-induced expression of early activation markers CD69, MHC class I and II, and of cyclin D of the PBLs.
Conclusions: These results are the first to indicate that RPE cells impede generation of activated T cells by interfering with the induction of high-affinity IL-2R-alphabetagamma, IL-2 production, and the expression of CD71 and cyclin A.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje