[Molecular identity of double-stranded RNA-elements of viral nature isolated from the sugar beet].

Autor: Mel'nichuk MD, Spiridonov VG
Jazyk: ruština
Zdroj: Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993) [Mikrobiol Z] 2005 Mar-Apr; Vol. 67 (2), pp. 55-62.
Abstrakt: Hybridization analysis of the double-strand RNA-elements isolated from the sugar beet of the Kyiv, Vinnytsya and Poltava Regions was carried out. A positive signal has been obtained with the dsRNA fragment, 1.9 kbp long, which codes the viral RNA- dependent RNA-polymerase having high level of homology with the analogous enzyme of mycoviruses from Partitiviridae family. Molecular identity of these dsRNA-elements in the sugar beet samples from the Vinnytsya and Poltava regions has been shown The RT-PCR test system has been developed for detecting the nondescribed mycovirus which is proposed to be called Helicobasidium purpureum partitivirus.
Databáze: MEDLINE