| Autor: |
Zeineddine D; Centre de Recherches de Biochimie Macromoléculaire, Montpellier, France., Papadimou E, Mery A, Ménard C, Pucéat M |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular medicine [Methods Mol Med] 2005; Vol. 112, pp. 175-82. |
| Abstrakt: |
Embryonic stem (ES) cells represent a source for cell-based regenerative therapies of heart failure. The pluripotency and the plasticity of ES cells allow them to be committed to a cardiac lineage following treatment with growth factors of the transforming growth factor (TGF)-beta superfamily. We describe a protocol designed to turn on expression of cardiac-specific genes in undifferentiated murine ES cells stimulated with BMP2 and/or TGF-beta. Cell commitment results in a significant improvement in spontaneous cardiac differentiation of ES cells both in vitro and in vivo. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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