[Biologic properties of an anti-human ovarian carcinoma/anti-human CD3 single chain bispecific antibody].
| Autor: | Yang JZ; Institute of Molecular Immunology, Southern Medical University, Guangzhou, Guangdong, 510515, PR China., Zhang Z, Ma L, Yao XS, Zhou MQ, Wang XB, Wang XN |
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| Jazyk: | čínština |
| Zdroj: | Ai zheng = Aizheng = Chinese journal of cancer [Ai Zheng] 2005 Jul; Vol. 24 (7), pp. 787-91. |
| Abstrakt: | Background & Objective: Previous routine therapies can' t improve the survival rate of ovarian carcinoma patients. Experimental and pre-clinical data showed that bispecific antibodies could efficiently induce antitumor effect of cytotoxic cells. This study was to investigate the biologic properties of an anti-human ovarian carcinoma/anti-human CD3 single chain bispecific antibody (BHL-I) in vitro, and provide reference for pre-clinical experiment and its application. Methods: Peripheral blood lymphocytes (PBLs), isolated from peripheral blood of healthy donors, were treated with BHL-I. The conjugation between PBLs and target ovarian carcinoma SKOV3 cells was observed under reverse microscope; the proliferation of peripheral blood mononuclear cells (PBMCs) and cytotoxicity of PBLs to SKOV3 cells were detected by MTT assay; the concentrations of human interferon-gamma (hIFN-gamma) and human tumor necrosis factor-alpha (hTNF-alpha), secreted by PBLs in the process of killing target cells, were detected by ELISA. Results: The rosette (PBL-SKOV3) formation rate was significantly higher in BHL-I group than in control group (15.7% vs. 11.1%, P<0.01). BHL-I significantly enhanced the proliferation of PBLs and cytotoxicity of PBLs to SKOV3 cells in the presence of relative antigen (P<0.01); the cytotoxic rate was positively correlated with the rosette formation rate (r=0.946); the concentrations of hIFN-gamma and hTNF-alpha were significantly increased (P<0.01). Conclusion: BHL-I could mediate conjugation between PBLs and SKOV3 cells, and activate the cytotoxicity of PBLs which may relate with up-regulation of hIFN-gamma and hTNF-alpha. |
| Databáze: | MEDLINE |
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