| Autor: |
Drew D; Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden., Slotboom DJ, Friso G, Reda T, Genevaux P, Rapp M, Meindl-Beinker NM, Lambert W, Lerch M, Daley DO, Van Wijk KJ, Hirst J, Kunji E, De Gier JW |
| Jazyk: |
angličtina |
| Zdroj: |
Protein science : a publication of the Protein Society [Protein Sci] 2005 Aug; Vol. 14 (8), pp. 2011-7. Date of Electronic Publication: 2005 Jun 29. |
| DOI: |
10.1110/ps.051466205 |
| Abstrakt: |
We describe a generic, GFP-based pipeline for membrane protein overexpression and purification in Escherichia coli. We exemplify the use of the pipeline by the identification and characterization of E. coli YedZ, a new, membrane-integral flavocytochrome. The approach is scalable and suitable for high-throughput applications. The GFP-based pipeline will facilitate the characterization of the E. coli membrane proteome and serves as an important reference for the characterization of other membrane proteomes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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