| Autor: |
Akiyoshi H; Laboratory of Veterinary Surgery, Department of Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai-shi, Osaka 599-8531, Japan., Aoki M, Kumagai D, Saleh N, Noda K, Shimada T, Sugii S, Ohashi F |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of veterinary medical science [J Vet Med Sci] 2005 May; Vol. 67 (5), pp. 535-7. |
| DOI: |
10.1292/jvms.67.535 |
| Abstrakt: |
Bovine and canine chromogranin A were extracted and purified from each specie's adrenal glands. Isolated bovine 70 kDa protein showed 100% identity to bovine CgA reported previously, whereas isolated canine 68 kDa protein showed 83.3% identity to bovine CgA by the NH(2)-terminal amino acid sequence analysis. Rabbit antibody to purified bovine protein (CgA) was found to immunologically cross-reacted with purified canine protein (CgA). In sandwich ELISA with anti-bovine CgA, concentration-dependent curves were obtained ranging from 0.3 to 20 mug/ml for canine CgA. From these findings, sandwich ELISA with anti-bovine CgA is found to be useful to determine the concentration of canine CgA. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
