| Autor: |
Rogers PH; Physics Department, California Polytechnic State University, San Luis Obispo, California 93407, USA., Michel E, Bauer CA, Vanderet S, Hansen D, Roberts BK, Calvez A, Crews JB, Lau KO, Wood A, Pine DJ, Schwartz PV |
| Jazyk: |
angličtina |
| Zdroj: |
Langmuir : the ACS journal of surfaces and colloids [Langmuir] 2005 Jun 07; Vol. 21 (12), pp. 5562-9. |
| DOI: |
10.1021/la046790y |
| Abstrakt: |
The directed three-dimensional self-assembly of microstructures and nanostructures through the selective hybridization of DNA is the focus of great interest toward the fabrication of new materials. Single-stranded DNA is covalently attached to polystyrene latex microspheres. Single-stranded DNA can function as a sequence-selective Velcro by only bonding to another strand of DNA that has a complementary sequence. The attachment of the DNA increases the charge stabilization of the microspheres and allows controllable aggregation of microspheres by hybridization of complementary DNA sequences. In a mixture of microspheres derivatized with different sequences of DNA, microspheres with complementary DNA form aggregates, while microspheres with noncomplementary sequences remain suspended. The process is reversible by heating, with a characteristic "aggregate dissociation temperature" that is predictably dependent on salt concentration, and the evolution of aggregate dissociation with temperature is observed with optical microscopy. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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