| Autor: |
Wan HM; Department of Chemical Engineering, National Tsing Hua University, Taiwan, Republic of China., Chen CC, Giridhar R, Chang TS, Wu WT |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of industrial microbiology & biotechnology [J Ind Microbiol Biotechnol] 2005 Jun; Vol. 32 (6), pp. 227-33. Date of Electronic Publication: 2005 May 14. |
| DOI: |
10.1007/s10295-005-0230-5 |
| Abstrakt: |
A cell-retention fermenter was used for the pilot-scale production of kojic acid using an improved strain of Aspergillus oryzae in repeated-batch fermentations. Among the various carbon and nitrogen sources used, sucrose and yeast extract promoted pellet morphology of fungi and higher kojic acid production. Repeated-batch culture using a medium replacement ratio of 75% gave a productivity of 5.3 gL(-1)day(-1) after 11.5 days of cultivation. While batch culture in shake-flasks resulted in a productivity of 5.1 gL(-1)day(-1), a productivity of 5 gL(-1)day(-1) was obtained in a pilot-scale fermenter. By converting the batch culture into repeated batches, the non-productive downtime of cleaning, filling and sterilizing the fermenter between each batch were eliminated, thereby increasing the kojic acid productivity. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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