| Autor: |
Zordan MA; Department of Human Anatomy and Physiology, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8620, Université Paris Sud, Orsay, France ., Massironi M, Ducato MG, Te Kronnie G, Costa R, Reggiani C, Chagneau C, Martin JR, Megighian A |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of neurophysiology [J Neurophysiol] 2005 Aug; Vol. 94 (2), pp. 1074-83. Date of Electronic Publication: 2005 May 04. |
| DOI: |
10.1152/jn.00954.2004 |
| Abstrakt: |
Vertebrate CASK is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. CASK is present in the nervous system where it binds to neurexin, a transmembrane protein localized in the presynaptic membrane. The Drosophila homologue of CASK is CAKI or CAMGUK. CAKI is expressed in the nervous system of larvae and adult flies. In adult flies, the expression of caki is particularly evident in the visual brain regions. To elucidate the functional role of CASK, we employed a caki null mutant in the model organism Drosophila melanogaster. By means of electrophysiological methods, we analyzed, in adult flies, the spontaneous and evoked neurotransmitter release at the neuromuscular junction (NMJ) as well as the functional status of the giant fiber pathway and of the visual system. We found that in caki mutants, when synaptic activity is modified, the spontaneous neurotransmitter release of the indirect flight muscle NMJ was increased, the response of the giant fiber pathway to continuous stimulation was impaired, and electroretinographic responses to single and continuous repetitive stimuli were altered and optomotor behavior was abnormal. These results support the involvement of CAKI in neurotransmitter release and nervous system function. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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