Birth of a healthy baby after transfer of blastocysts derived from cryopreserved human oocytes fertilized with frozen spermatozoa.
| Autor: | Tjer GC; Department of Obstetrics and Gynecology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin NT, Hong Kong SAR, China., Chiu TT, Cheung LP, Lok IH, Haines CJ |
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| Jazyk: | angličtina |
| Zdroj: | Fertility and sterility [Fertil Steril] 2005 May; Vol. 83 (5), pp. 1547-9. |
| DOI: | 10.1016/j.fertnstert.2005.01.007 |
| Abstrakt: | Objective: To report the birth of a healthy baby after transfer of blastocysts derived from frozen eggs and frozen spermatozoa. Design: Case report. Setting: University-based assisted reproduction center. Patient(s): A 37-year-old woman with secondary infertility of 4 years' duration. Intervention(s): Retrieved oocytes were cryopreserved in 1.5 M 1,2-propanediol (PROH) and 0.3 M sucrose by a slow freezing-rapid thawing protocol, semen cryopreservation, and insemination by intracytoplasmic sperm injection (ICSI) at 4 hours after thawing. Main Outcome Measure(s): Fertilization and embryo development to blastocyst stage, pregnancy, and outcome. Result(s): Ten of 14 frozen oocytes survived after thawing. Eight of them were fertilized by performing ICSI and three developed into 7- to 8-cell embryos on day 3. Two of these embryos developed into blastocysts on day 5 and were transferred. This resulted in a successful pregnancy and the delivery of a healthy baby boy. Conclusion(s): This case demonstrates the feasibility of inseminating the frozen-thawed human oocytes after 4 hours of in vitro culture and the zygotes derived from frozen oocytes and frozen spermatozoa can be cultured to blastocysts resulting in the live birth of a healthy baby boy. |
| Databáze: | MEDLINE |
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