| Autor: |
Melles E; Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden., Anderson H, Wallinder D, Shafqat J, Bergman T, Aastrup T, Jörnvall H |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical biochemistry [Anal Biochem] 2005 Jun 01; Vol. 341 (1), pp. 89-93. |
| DOI: |
10.1016/j.ab.2005.03.005 |
| Abstrakt: |
Proinsulin C-peptide was electroimmobilized to a quartz crystal microbalance sensor chip, localizing this low-pI peptide for covalent attachment to activated surface carboxyl groups. The resulting chip was used in a continuous flow biosensor to capture anti-C-peptide antibodies, which could subsequently be eluted in 5% formic acid between air bubbles for efficient recovery and mass spectrometric identification. The method is reproducible through repeated cycles, providing affinity purification of proteins under real-time monitoring of the binding and elution processes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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