| Autor: |
Winters MA; Center for AIDS Research, Stanford University, Stanford, CA, USA. mark.winters@stanford.edu, Kagan RM, Kovari L, Heseltine PN, Merigan TC |
| Jazyk: |
angličtina |
| Zdroj: |
Antiviral therapy [Antivir Ther] 2005; Vol. 10 (2), pp. 363-6. |
| Abstrakt: |
HIV-1 strains that possess a one or two amino acid insert between codons 102 and 103 of the reverse transcriptase (RT) gene were identified in three HIV-1-infected individuals. Each strain also had one or more known mutations associated with nucleoside RT inhibitors (NRTIs) and non-nucleoside RT inhibitors (NNRTIs). Recombinant viruses from these strains had reduced susceptibility to efavirenz and nevirapine, and homology modelling predicted a loss of binding contacts with efavirenz. Mutagenesis studies indicated that replication of insert-containing strains was dependent on RT gene mutations and polymorphisms that co-evolved with the insert. These results suggest that inserts in the NNRTI-binding pocket contribute to NNRTI resistance, but are tolerated only under specific genetic conditions. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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