Bile duct ligation induced acute inflammation up-regulates cyclooxygenase-2 content and PGE2 release in guinea pig gallbladder smooth muscle cell cultures.

Autor: Myers SI; Department of Surgery, Virginia Commonwealth University, 1200 E. Broad St., West Hospital, Ninth Floor, Richmond, VA 23298-0108, USA. simyers@vcu.edu, Bartula LL, Colvin MP, Parkman HP, Braverman AA, Ruggieri MR
Jazyk: angličtina
Zdroj: Prostaglandins, leukotrienes, and essential fatty acids [Prostaglandins Leukot Essent Fatty Acids] 2005 May; Vol. 72 (5), pp. 327-33.
DOI: 10.1016/j.plefa.2004.12.005
Abstrakt: Introduction: This study examines hypotheses that BDL induces increased guinea pig gallbladder smooth muscle PGE2 release by up-regulation of COX-2.
Methods: BDL, Sham and Control Hartley guinea pig gallbladders were placed in cell culture, grown to confluence and underwent Western Blot analysis for smooth muscle cell content of COX-1, COX-2, Prostacylin Synthase, actin, caldesmon, vinculin, meta-vinculin and tropomyosin and were assayed for basal release of 6-keto-PGF(1alpha), PGE2 and TxB2 by EIA.
Results: BDL did not alter content of smooth muscle cytoskeletal proteins. BDL for 48 h increased smooth muscle cell release of PGE2 and 6-keto-PGF(1alpha) by 3-fold or more when compared to the Control and Sham groups. Western Blot analysis showed increased content of COX-2 in the BDL group.
Conclusions: BDL for 48 h markedly increased endogenous guinea pig smooth muscle cell PG release, which was due to increased COX-2 synthesis.
Databáze: MEDLINE
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