| Autor: |
Aguirre SR; Instituto de Medicina Tropical 'Pedro Kouri' Ciudad de La Habana, Cuba. sresik@ipk.sld.cu, Ping LH, Barrios Olivera JA, Cardellá VK, Swanstrom R |
| Jazyk: |
Spanish; Castilian |
| Zdroj: |
Revista cubana de medicina tropical [Rev Cubana Med Trop] 2003 Sep-Dec; Vol. 55 (3), pp. 213-6. |
| Abstrakt: |
The efficiency of SensiScript reverse transciptase to obtain useful genetic material in the sequencing of the nucleic acid from HIV-1, starting from sera collected between 1989 and 1998 and kept at suboptimal temperatures, was proved. On using the SensiScript enzyme it was obtained an amplification of the RNA of the HIV-1 in 86.5 % of the studied samples, compared with 20 % on using the AMV-RT enzyme . No amplification was obtained in 13.5 % of the studied samples with any of the 2 enzymes used. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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