Identification of a novel insulin-like growth factor binding protein gene homologue with tumor suppressor like properties.

Autor: Cai Z; Nuvelo, Inc., 675 Almanor Ave., Sunnyvale, CA 94085, USA., Chen HT, Boyle B, Rupp F, Funk WD, Dedera DA
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2005 May 27; Vol. 331 (1), pp. 261-6.
DOI: 10.1016/j.bbrc.2005.03.163
Abstrakt: Here we report the identification of a new insulin-like growth factor binding protein homologue, provisionally designated insulin-like growth factor binding related protein-4 (IGFBP-rP4). IGFBP-rP4 was found to be most closely related to IGFBP-7 with 52% amino acid homology and 43% amino acid identity, and shares a similar domain structure. Semi-quantitative RT-PCR expression analysis demonstrated a pattern of downregulation of this gene in multiple tumor samples including lung and colon cancer, compared to matched adjacent normal tissue. Western blotting revealed a protein of approximately 38kDa expressed in both the cell pellet and secreted into the supernatant of transiently transfected Cos-7 cells. Cos-7 supernatants containing IGFBP-RP4 protein were observed to suppress the growth of HeLa cells in culture compared to vector controls. IGFBP-RP4 directly transiently transfected into HeLa cells also further confirmed the growth suppressive properties of this protein. Together these data suggest that IGFBP-RP4 may be a novel putative tumor suppressor protein.
Databáze: MEDLINE
Externí odkaz: