| Autor: |
Bezuglyĭ SV, Skripal' IG, Babichev VV |
| Jazyk: |
ruština |
| Zdroj: |
Mikrobiologicheskii zhurnal [Mikrobiol Zh (1978)] 1992 Jan-Feb; Vol. 54 (1), pp. 51-7. |
| Abstrakt: |
The DNA-dependent DNA-polymerase (DNA polymerase I which is not sorbed on the column with DEAE-cellulose, and DNA-polymerase II, which is absorbed by this column and is eluted from it by 0.3 M of NaCl), have been isolated from Acholeplasma laidlawii PG-8. DNA-polymerase I in homogeneous state was obtained as a result of the stepwise treatment by heparin-sepharose (elution at 0.35 M of NaCl) and poly-U-sepharose (elution at 0.3 M of NaCl). It was presented on the electrophoregram by one polypeptide with molecular weight of 72 kDalton. The second form of DNA polymerase was also obtained in homogeneous state as a result of sequential treatment on heparin-sepharose (elution at 0.3 M of NaCl) and on poly-A-sepharose (elution at 0.25 M of NaCl): the protein which had manifested polymerase activity was a polypeptide with molecular weight of 45 kDalton. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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