| Autor: |
Goodwin AE; Department of Fisheries and Allied Aquacultures, Alabama Agricultural Experimental Station, Auburn University 36849., Grizzle JM, Bradley JT, Estridge BH |
| Jazyk: |
angličtina |
| Zdroj: |
Comparative biochemistry and physiology. B, Comparative biochemistry [Comp Biochem Physiol B] 1992 Mar; Vol. 101 (3), pp. 441-6. |
| DOI: |
10.1016/0305-0491(92)90025-m |
| Abstrakt: |
1. A monoclonal antibody to vitellogenin of channel catfish (Ictalurus punctatus) was made, and its specificity was demonstrated using Western blots of serum from female fish, estradiol-treated male fish, untreated male fish, vitellogenin purified by three different methods and egg extracts. 2. An enzyme-linked immunosorbent assay (ELISA), using this monoclonal antibody, detected vitellogenin in the plasma of 59 out of 60 untreated 17-24-month-old male channel catfish with a mean concentration of 338 micrograms/ml and a maximum concentration of 4240 micrograms/ml. 3. Vitellogenin levels in male channel catfish were unrelated to testicular stage, gonadosomatic index and month. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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