| Autor: |
Belyĭ IuF, Sheklakova LA, Vakhrameeva MS, Zhukhovitskiĭ VG, Petrukhin VG, Belaia IuA |
| Jazyk: |
ruština |
| Zdroj: |
Molekuliarnaia genetika, mikrobiologiia i virusologiia [Mol Gen Mikrobiol Virusol] 2005 (1), pp. 32-5. |
| Abstrakt: |
Vacuolizing toxin (VacA) Helicobacter pylori is an important factor of pathogenicity of Helicobacter pylori and a basic marker in the diagnosis of helicobacteriosis and related diseases. A coagulation-based diagnostic test-system was elaborated for the detection of VacA in clinical samples. A fragment of vacA was cloned, for the purpose, in Escherichia coli and expressed in preparative quantities; the coded protein was purified and used in raising the diagnostic serum. The thus designed coagulating test-system was successfully tested under the modeling conditions with clinical samples. Therefore, the designed express method can be used for the invasion-free determination of VacA in patients with gastric and duodenal pathologies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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