| Autor: |
Sultan M; National Organization for Drug Control and Research, Cairo, Egypt., Stecher G, Stöggl WM, Bakry R, Zaborski P, Huck CW, El Kousy NM, Bonn GK |
| Jazyk: |
angličtina |
| Zdroj: |
Current medicinal chemistry [Curr Med Chem] 2005; Vol. 12 (5), pp. 573-88. |
| DOI: |
10.2174/0929867310504050573 |
| Abstrakt: |
The article discusses the qualitative and quantitative determination of non-steroidal anti-inflammatory drugs like salicin, salicylic acid, tenoxicam, ketorolac, piroxicam, tolmetin, naproxen, flurbiprofen, diclofenac and ibuprofen by reversed phase high performance liquid chromatography (RP-HPLC) and micro-HPLC (micro-HPLC) hyphenated with UV-absorbance and mass spectrometric detection. Both detection methods delivered calibration plots with good linearity (r(2) > 0.9800), limits of detection in the low nanogram range and recovery rates between 94 and 104 %. For the analysis of biological samples such as blood, plasma and erythrocytes liquid-liquid extraction (LLE) and solid phase extraction (SPE) on the basis of new synthesized glycidylmethacrylate/divinylbenzene copolymer (GMA/DVB) particles and commercially available material on the basis of poly(divinylbenzene-co-N-vinylpyrrolidone) copolymer were investigated. Finally the use of a micro-HPLC system with separation columns in the range of 8 cm x 200 microm I.D. for the determination of non-steroidal anti-inflammatory drugs (NSAIDs) is presented, emphasizing on the type of column and sample amount needed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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