[High cell density fed-batch culture of E.coli expressing rhVEGF121].

Autor:	Hu ZM; Institute of Molecular Immunology, Southern Medical University, Guangzhou 510515, China. hzm@fimmu.com, Ma L, Zhou MQ, Meng MJ, Yang JZ, Wang XN
Jazyk:	čínština
Zdroj:	Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA [Di Yi Jun Yi Da Xue Xue Bao] 2005 Mar; Vol. 25 (3), pp. 267-9.
Abstrakt:	Objective: To investigate the optimal high cell density fermentation conditions of recombinant E.coli BL21/pET- 24a/hVEGF(121) expressing recombinant human vascular endothelial growth factor (rhVEGF(121)). Methods: The effects of the composition of the fermentation medium, induction time and fed-batch carbon sources on the expression level of rhVEGF(121) and cell output were analyzed. Results and Conclusion: When cultured in modified M9 medium and induced for 4 h in the presence of 0.5 mmol/L IPTG at 37 degrees celsius; with glycerol as the carbon sources by continuous fed-batch mode, the recombinant E.coli expressed rhVEGF(121) at the level up to 23% of the total proteins and the yield reached 68 g/L. The optimized fermentation condition for recombinant E.coli enables high expression level of rhVEGF(121).
Databáze:	MEDLINE
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