Variability of ribosomal RNA genes in Rauwolfia species: parallelism between tissue culture-induced rearrangements and interspecies polymorphism.

Autor: Andreev IO; Institute of Molecular Biology and Genetics, National Academy of Science of Ukraine, Academy Zabolotnogo Street, 150, Kiev-143, 03143 Ukraine. i.o.andreev@imbg.org.ua, Spiridonova KV, Solovyan VT, Kunakh VA
Jazyk: angličtina
Zdroj: Cell biology international [Cell Biol Int] 2005 Jan; Vol. 29 (1), pp. 21-7. Date of Electronic Publication: 2005 Jan 20.
DOI: 10.1016/j.cellbi.2004.11.002
Abstrakt: An analysis of 18S-25S and 5S rRNA genes in intact plants and cultured tissues of some Rauwolfia species was performed to compare these sequences variability occurred as a result of the species evolution in nature and that induced by tissue culture. The restriction fragment length polymorphism of 18S-25S and 5S rDNA was found both in intact plants of various Rauwolfia species and in long-term Rauwolfia serpentina tissue cultures. In addition, changes in the amount of 18S-25S rRNA genes were observed in long-term R. serpentina tissue cultures. The results demonstrate that rDNA variability observed in intact plants as well as in long-term cultures is attributed to differences in the same regions of ribosomal RNA genes.
Databáze: MEDLINE