| Autor: |
Nacher V; Department of Animal Health and Anatomy and Center for Animal Biotechnology and Gene Therapy (CBATEG), Autonomous University of Barcelona, Spain., Carretero A, Navarro M, Armengol C, Llombart C, Blasi J, Ruberte J |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of anatomy [J Anat] 2005 Feb; Vol. 206 (2), pp. 165-74. |
| DOI: |
10.1111/j.1469-7580.2005.00382.x |
| Abstrakt: |
beta-Catenin is a structural component of adherens junctions, a regulator of the Wnt signalling pathway and a transcriptional co-activator with a key role in vascular patterning. The avian mesonephros is a transitory embryonic kidney that is used in the study of vascular development and degeneration. Here we examine beta-catenin expression in this model during vascular development and degeneration. Quail embryos with developing or degenerating mesonephros were studied, on day 6 (30HH) or day 11 of incubation (40HH), respectively. QH1 whole mounts of developing mesonephros revealed numerous angioblast-like cells situated in the paramesonephric duct that seem to invade the mesonephros. Although these cells did not express beta-catenin, the surrounding periductal mesenchymal cells translocated high levels of beta-catenin into the nucleus. In contrast, degenerating mesonephros were devoid of angioblast-like cells and beta-catenin was lower than in the developing mesonephros. beta-Catenin was significantly reduced in the glomerular capillary tuffs, indicating that it was particularly down-regulated in the vascular system. No sex-related differences in beta-catenin expression were observed in degenerating mesonephros. Furthermore, two special populations of glomerular and peritubular endothelial cells were observed in degenerating mesonephros: one translocating beta-catenin into the nucleus and the other in apoptosis that did not translocate it. In conclusion, our results indicate that the paramesonephric duct is a potential new vasculogenetic pathway, and suggest that beta-catenin plays a role in the fate of mesonephric endothelial cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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