| Autor: |
Rush GJ; Institute of Infectious Diseases and Molecular Medicine, Health Sciences Faculty, University of Cape Town, Observatory, 7925, Cape Town, South Africa. grush@curie.uct.ac.za, Steyn LM |
| Jazyk: |
angličtina |
| Zdroj: |
Biotechnology letters [Biotechnol Lett] 2005 Feb; Vol. 27 (3), pp. 173-9. |
| DOI: |
10.1007/s10529-004-7664-0 |
| Abstrakt: |
The effect on translation of downstream box sequences optimized for binding to Mycobacterium smegmatis and Escherichia coli 16S rRNA in the absence of a Shine-Dalgarno (SD) region was investigated. The relative translational efficiency of each construct in either M. smegmatis or E. coli was determined. Eradication of the SD region in the absence of a downstream box abolished the translation activity. In contrast, optimized downstream box constructs resulted in a 13- and 18-fold increase in protein synthesis, relative to non-optimized DB controls in E. coli and M. smegmatis, respectively. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full text from SpringerLink