| Autor: |
Zhang B; University of Maryland Greenebaum Cancer Center, University of Maryland Medical System, Baltimore, MD 21201, USA., Arany Z, Mann D, Rhee JG, Fenton RG |
| Jazyk: |
angličtina |
| Zdroj: |
Apoptosis : an international journal on programmed cell death [Apoptosis] 2005 Jan; Vol. 10 (1), pp. 219-31. |
| DOI: |
10.1007/s10495-005-6077-4 |
| Abstrakt: |
Apoptosis is characterized by chromatin condensation, DNA cleavage, redistribution of phosphatidylserine, and apoptotic body formation via an actin-dependent process. We describe a novel form of the execution phase of apoptosis in human multiple myeloma cells that is morphologically and mechanistically distinct from classical apoptosis, but is caspase-dependent and inhibited by IL-6 and overexpression of Bcl-2. Electron microscopic analysis of these cells demonstrated chromatin condensation without nuclear fragmentation, and 'partitioning' of cell constituents into two components: a single, large bleb containing soluble protein and free ribosomes, and a region containing the nucleus, organelles, and RER. In some cases, the bleb separated, becoming a free vesicle exhibiting random kinetic motion. These morphologic features occurred despite inhibition of the actin and tubulin cytoskeletal systems. This novel form of apoptosis, called partitioning apoptosis, was observed in a variety of tumor cell types and in primary cells. The execution phase of apoptosis can occur in a manner that is morphologically and mechanistically distinct from classical apoptosis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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