| Autor: |
Borgnon J; INSERM EMI 00-20 and University Paris XI, Bicêtre Hospital, Kremlin-Bicêtre, France., Djamouri F, Lorand I, Rico VD, Loux N, Pages JC, Franco D, Capron F, Weber A |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2005 Mar 25; Vol. 328 (4), pp. 937-43. |
| DOI: |
10.1016/j.bbrc.2005.01.049 |
| Abstrakt: |
Retroviral vectors are widely used tools for gene therapy. However, in vivo gene transfer is only effective in dividing cells, which, in liver, requires a regenerative stimulus. Follistatin is effective in promoting liver regeneration after 90% and 70% hepatectomy in rats. We studied its efficacy on liver regeneration and retroviral-mediated gene delivery in 50% hepatectomized rats. When human recombinant follistatin was infused into the portal vein immediately after 50% hepatectomy, hepatocyte proliferation was significantly higher than in control 50% hepatectomized rats. A single injection of virus particles administered 23 h after follistatin infusion resulted in more than 20% gene transduction efficiency in hepatocytes compared to 3% in control rats. It is concluded that a single injection of follistatin induces onset of proliferation in 50% hepatectomized rats and allows efficient retroviral-mediated gene transfer to the liver. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full Text from ScienceDirect