Three-dimensional chemical structures by protein functionalized micron-sized beads bound to polylysine-coated silicone surfaces.

Autor: Lateef SS; Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois 60607, USA., Boateng S, Ahluwalia N, Hartman TJ, Russell B, Hanley L
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2005 Mar 15; Vol. 72 (4), pp. 373-80.
DOI: 10.1002/jbm.a.30229
Abstrakt: A novel method is described here that allows three-dimensional (3D) control of both chemistry and morphology by a series of wet chemical steps: the attachment of protein functionalized micron-sized beads onto a flat silicone surface that has been functionalized with a distinct chemical modification. Bovine serum albumin (BSA), laminin, or polylysine is covalently bound to 6.5-microm-diameter spherical beads. A chemical method is then used to bind these beads to a flat silicone surface that is subsequently functionalized with polylysine. This process leads to a nonspecific cell adhesive background on the flat surface (polylysine) with the option of differing chemistry on the third-dimension due to the protein BSA or laminin on the bead protruding from the surface. The beads do not detach during cyclic stretching in vitro. Neo-natal rat cardiac fibroblasts are cultured on the beaded surfaces and compared with fibroblasts cultured on nonbeaded, flat polylysine surfaces. Fibroblast plating density, integrin, and physical responses are examined as a function of varying the ligands on the beads.
(Copyright (c) 2005 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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