Matrix-assisted ultraviolet laser desorption/ionization time-of-flight mass spectrometry of beta-(1 --> 3), beta-(1 --> 4)-xylans from Nothogenia fastigiata using nor-harmane as matrix.
| Autoři: | Fukuyama Y; Plant Biophysics/Biochemistry Research Laboratory, College of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan., Kolender AA, Nishioka M, Nonami H, Matulewicz MC, Erra-Balsells R, Cerezo AS |
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| Zdroj: | Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2005; Vol. 19 (3), pp. 349-58. |
| Způsob vydávání: | Journal Article; Research Support, Non-U.S. Gov't |
| Jazyk: | English |
| Informace o časopise: | Publisher: John Wiley And Sons Ltd Country of Publication: England NLM ID: 8802365 Publication Model: Print Cited Medium: Print ISSN: 0951-4198 (Print) Linking ISSN: 09514198 NLM ISO Abbreviation: Rapid Commun Mass Spectrom Subsets: MEDLINE |
| Imprint Name(s): | Publication: Chichester : John Wiley And Sons Ltd Original Publication: London, UK : Heyden, c1987- |
| Výrazy ze slovníku MeSH: | Carbolines/*chemistry , Harmine/*analogs & derivatives , Harmine/*chemistry , Seaweed/*chemistry , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods , Xylans/*analysis |
| Abstrakt: | Three xylan fractions isolated from the red seaweed Nothogenia fastigiata (Nemaliales) were analyzed by ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (UV-MALDI-TOFMS). UV-MALDI-TOFMS was carried out in the linear and reflectron modes, and as routine in the positive and negative ion modes. Of the several matrices tested, nor-harmane was the only effective one giving good spectra in the positive ion mode. The number-average molar masses of two of the fractions, calculated from the distribution profiles, were lower than those determined previously by (1)H NMR analysis, suggesting a decrease in the ionization efficiency with increasing molecular weight; weight-average molar mass and polydispersity index were also determined. As the xylans retained small but significant quantities of calcium salts, the influence of added Ca(2+) as CaCl(2) on UV-MALDI-MS was investigated. The simultaneous addition of sodium chloride and calcium chloride was also analyzed. Addition of sodium chloride did not change the distribution profile of the native sample showing that the inhibitory effect is due to Ca(2+) and not to Cl(-). Addition of calcium chloride with 1:1 analyte/salt molar ratio gave spectra with less efficient desorption/ionization of oligomers; the signals of these oligomers were completely suppressed when the addition of the salt became massive (1:100 analyte/salt molar ratio). (Copyright (c) 2005 John Wiley & Sons, Ltd.) |
| Substance Nomenclature: | 0 (Carbolines) 0 (Xylans) 4FHH5G48T7 (Harmine) 82D6J0535P (harman) |
| Entry Date(s): | Date Created: 20050113 Date Completed: 20050422 Latest Revision: 20131121 |
| Update Code: | 20221213 |
| DOI: | 10.1002/rcm.1783 |
| PMID: | 15645484 |
| Autor: | Fukuyama Y; Plant Biophysics/Biochemistry Research Laboratory, College of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan., Kolender AA, Nishioka M, Nonami H, Matulewicz MC, Erra-Balsells R, Cerezo AS |
| Jazyk: | angličtina |
| Zdroj: | Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2005; Vol. 19 (3), pp. 349-58. |
| DOI: | 10.1002/rcm.1783 |
| Abstrakt: | Three xylan fractions isolated from the red seaweed Nothogenia fastigiata (Nemaliales) were analyzed by ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (UV-MALDI-TOFMS). UV-MALDI-TOFMS was carried out in the linear and reflectron modes, and as routine in the positive and negative ion modes. Of the several matrices tested, nor-harmane was the only effective one giving good spectra in the positive ion mode. The number-average molar masses of two of the fractions, calculated from the distribution profiles, were lower than those determined previously by (1)H NMR analysis, suggesting a decrease in the ionization efficiency with increasing molecular weight; weight-average molar mass and polydispersity index were also determined. As the xylans retained small but significant quantities of calcium salts, the influence of added Ca(2+) as CaCl(2) on UV-MALDI-MS was investigated. The simultaneous addition of sodium chloride and calcium chloride was also analyzed. Addition of sodium chloride did not change the distribution profile of the native sample showing that the inhibitory effect is due to Ca(2+) and not to Cl(-). Addition of calcium chloride with 1:1 analyte/salt molar ratio gave spectra with less efficient desorption/ionization of oligomers; the signals of these oligomers were completely suppressed when the addition of the salt became massive (1:100 analyte/salt molar ratio). (Copyright (c) 2005 John Wiley & Sons, Ltd.) |
| Databáze: | MEDLINE |
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