| Autor: |
Szpechcinski A; Medical University and University Hospital, Bydgoszcz, Poland., Trzos R, Jarocki P, Trojan LA, Oficjalska K, Junkiert A, Wei MX, Mazurek M, Czapiewska JL, Niklinski J, Kopiński P, Chyczewski L, Kasacka I, Trojan J |
| Jazyk: |
angličtina |
| Zdroj: |
Roczniki Akademii Medycznej w Bialymstoku (1995) [Rocz Akad Med Bialymst] 2004; Vol. 49 Suppl 1, pp. 98-104. |
| Abstrakt: |
The supposed immunogenic character of glioma cells transfected with antisense IGF-I-Receptor (IGF-I-R) expression vector was tested for the presence of MHC-I currently present in cells of IGF-I antisense type. C6 rat glioma cell line was comparatively transfected in vitro with IGF I antisense (pMT-Anti-IGF I) or IGF I Receptor antisense (pMT-Anti-IGF I R) expression vectors. The wild and transfected cells were examined for the presence of IGF-I and MHC-I molecules. Using RT PCR technique, the transfected "antisens" cells showed total inhibition of IGF-I. The both transfected cultures of IGF-I and of IGF-I-R type were positively stained for MHC-I. Moreover "antisense IGF-I-R" cells as compared to "IGF-I antisense" cells showed slightly higher expression of MHC-I. The transfected cells showed also the feature of apoptosis in 60% of cells. The immunogenicity of IGF-I-R antisense glioma cells is related to MHC-I presence; therefore both approaches of antisense IGF-I and of antisense IGF-I-R could be use in paralel for cellular therapy of glioblastoma. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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