Nitric oxide and fetal organ blood flow during normoxia and hypoxemia in endotoxin-treated fetal sheep.

Autor:	Coumans AB; Department of Obstetrics and Gynecology, University of Maastricht, Maastricht, The Netherlands., Garnier Y, Supçun S, Jensen A, Berger R, Hasaart TH
Jazyk:	angličtina
Zdroj:	Obstetrics and gynecology [Obstet Gynecol] 2005 Jan; Vol. 105 (1), pp. 145-55.
DOI:	10.1097/01.AOG.0000146640.45530.69
Abstrakt:	Objective: To investigate the role of nitric oxide in the process of circulatory decentralization during fetal hypoxemia. Methods: Fifteen sheep with singleton pregnancies were chronically instrumented at 107 days of gestation (term is 147 days). Three days later, 8 of the fetuses received nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide synthesis. Fifteen minutes after L-NAME administration, all 15 fetuses received lipopolysaccharides (LPS) from a strain of Escherichia coli. The 7 fetuses that received LPS only were used as controls. Sixty minutes after LPS was administered, the maternal aorta was occluded for 2 minutes in all fetuses. Organ blood flow and physiological variables were measured at 75 minutes before the start of occlusion (ie, at the time of L-NAME administration to the experimental group), at 1 minute before the start of occlusion, and at 2, 4, and 30 minutes after the start of occlusion. Results: Arterial pH was lower in the L-NAME group than in the control group at 1 minute before and 2 minutes after occlusion. Mean arterial pressure was higher in the L-NAME group than in the control group at 2 and 4 minutes after occlusion. Cardiac output fell in the L-NAME group and was lower than in the control group; the percentage of cardiac output to the cerebrum in the L-NAME group was 35% lower than that in the control group. Throughout the study, placental blood flow decreased by more than 80% in both groups and remained low. Blood flow to the fetal body decreased by 65% in the L-NAME group and was lower than in the control group. Blood flow to the carcass also decreased in the L-NAME group and was 36% of that in the control group. Conclusion: Inhibition of nitric oxide synthesis causes a general vasoconstriction in practically all organs and leads to a reduction in LPS-induced circulatory decentralization. The changes in blood flow distribution in endotoxin-treated fetal sheep seem to be mediated in part by nitric oxide.
Databáze:	MEDLINE
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