| Autor: |
Gryzunov IuA, Komarova MN |
| Jazyk: |
ruština |
| Zdroj: |
Biofizika [Biofizika] 2004 Nov-Dec; Vol. 49 (6), pp. 979-86. |
| Abstrakt: |
A method for studing the binging of ligands absorbing the light in the region of 350-550 nm to protein is described. The method is based on resonance energy transfer between the fluorescent label covalently bound to the protein and the ligand. The isoindole label, a product of the reaction of the protein with o-phthalaldehyde in the presence of 2-mercaptoethanol, was used as a fluorescent donor. The method was used to determine the binding parameters of a fluorescent probe (a naphthalimide derivative) with human serum albumin. A comparison of the results obtained by the resonance energy and transfer by equilibrium dialysis showed a high accuracy of the resonance energy transfer method. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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