| Autor: |
Rudinskiĭ NI, Mikhaĭlovich VM, Donnikov MIu, Lapa SA, Sukhanova AL, Kazennova EV, Bobkov AF, Zasedatelev AS, Pokrovskiĭ VV, Mirzabekov AD |
| Jazyk: |
ruština |
| Zdroj: |
Voprosy virusologii [Vopr Virusol] 2004 Nov-Dec; Vol. 49 (6), pp. 10-5. |
| Abstrakt: |
An original biochip was constructed for the detection of 34 mutations of HIV-1 resistance to protease. A technology was worked out, which is based on the hybridization of a fluorescence-labeled amplified fragment of the pol gene of the HIV-1 provirus DNA with a set of specific oligonucleotides immobilized in 3-D hydrogel pads of the biological microchip. The biochip was used to analyze 115 samples of the subtype-1 provirus HIV-1 DNA isolated from untreated IDUs and their sexual partners in 15 regions of former USSR countries. Substitution of Val/IIe in position 77 of protease (V771) is known as secondary mutation of resistance to Nelfinavir detected in 55 (47.8%) of 115 HIV-1 variations. Its first appearance was registered in a patient with HIV in April 1997 in Tver, where its carrying variant caused an HIV outbreak. It is demonstrated that the V771-substitution variant, that dominates in Moscow, caused outbreaks in Irkutsk and Yekaterinburg and spread into separate districts of Perm and Perm Region. At the same time, no V771 HIV-1 was detected in any of the HIV studied cases diagnosed before 1998 in Moldova, Ukraine and Rostov Region. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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