Multiple sampling for increasing the diagnostic sensitivity of nipple aspirate fluid for atypical cytology.
| Autor: | King EB; Department of Epidemiology and Biostatistics, School of Medicine, University of California San Francisco, San Francisco, California 94143-0560, USA., Chew KL, Hom JD, Miike R, Wrensch MR, Petrakis NL |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Acta cytologica [Acta Cytol] 2004 Nov-Dec; Vol. 48 (6), pp. 813-7. |
| DOI: | 10.1159/000326451 |
| Abstrakt: | Objective: To determine if repeated collection of nipple aspirate fluid (NAF) can improve the diagnostic sensitivity for cytologic atypia, a marker of increased risk of breast cancer. Study Design: Two hundred sixty-seven women without known breast disease volunteered for NAF cytology at 5 6-month intervals over 2 years. NAF samples were prepared on Millipore filters (Millipore Filter Corp., Bedford, Massachusetts, U.S.A.) and stained with a modified Papanicolaou method. Fluid availability and cellular abnormalities were evaluated for each collection attempt. Cellular findings were classified as benign, hyperplasia or atypia. Results: NAF was obtained from 178 women (66.6%) at the first visit and from an additional 15, 10, 2 and 4 women at visits 2, 3, 4 and 5, respectively, for a cumulative total of 78.2% by visit 5. The number of women yielding NAF containing hyperplastic or atypical epithelial cells was determined at each visit. Hyperplastic cells were found in 34 (19.1%) at visit 1 and in an additional 20, 10, 5 and 4 women at visits 2, 3, 4 and 5, respectively. Atypical epithelial cells were present in 12 (6.7%) women at the initial visit and in an additional 11, 7, 5 and 1 women at visits 2, 3, 4 and 5, respectively, for a cumulative percent of 18.2 at visit 5. NAF could not be obtained from 58 women at any visit. Conclusion: These findings suggest that an optimum collection method for NAF cytology should consist of at least 3 or 4 separate fluid aspiration attempts. Reviewing repeated multiple samples instead of 1 increases the number of women who can be evaluated and the likelihood of detecting cytologic atypia. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|