| Autor: |
Elshahed MS; Department of Botany and Microbiology, and Institute of Energy and the Environment, University of Oklahoma, Norman, OK 73019, USA., Savage KN; Department of Botany and Microbiology, and Institute of Energy and the Environment, University of Oklahoma, Norman, OK 73019, USA., Oren A; The Institute of Life Sciences and the Moshe Shilo Minerva Center for Marine Biogeochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel., Gutierrez MC; Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Seville, Spain., Ventosa A; Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Seville, Spain., Krumholz LR; Department of Botany and Microbiology, and Institute of Energy and the Environment, University of Oklahoma, Norman, OK 73019, USA. |
| Abstrakt: |
A pleomorphic, extremely halophilic archaeon (strain M6(T)) was isolated from a sulfide- and sulfur-rich spring in south-western Oklahoma (USA). It formed small (0.8-1.0 mm), salmon pink, elevated colonies on agar medium. The strain grew in a wide range of NaCl concentrations (6 % to saturation) and required at least 1 mM Mg(2+) for growth. Strain M6(T) was able to reduce sulfur to sulfide anaerobically. 16S rRNA gene sequence analysis indicated that strain M6(T) belongs to the family Halobacteriaceae, genus Haloferax; it showed 96.7-98.0 % similarity to other members of the genus with validly published names and 89 % similarity to Halogeometricum borinquense, its closest relative outside the genus Haloferax. Polar lipid analysis and DNA G+C content further supported placement of strain M6(T) in the genus Haloferax. DNA-DNA hybridization values, as well as biochemical and physiological characterization, allowed strain M6(T) to be differentiated from other members of the genus Haloferax. A novel species, Haloferax sulfurifontis sp. nov., is therefore proposed to accommodate the strain. The type strain is M6(T) (=JCM 12327(T)=CCM 7217(T)=DSM 16227(T)=CIP 108334(T)). |
