| Autor: |
Barnard DE; Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA., Sampugna J, Berlin E, Bhathena SJ, Knapka JJ |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of nutritional biochemistry [J Nutr Biochem] 1990 Apr; Vol. 1 (4), pp. 190-5. |
| DOI: |
10.1016/0955-2863(90)90105-t |
| Abstrakt: |
The substitution of trans- for half of the cis-monounsaturated fatty acids in the diet of Macaca fasicularis monkeys resulted in alterations in erythrocyte fatty acid composition and insulin receptor properties but not in membrane fluidity. Both cis and trans diets contained 10% fat and similar fatty acid compositions, except that approximately 50% of the cis-octadecenoate (c-18:1) in the cis diet was replaced with trans-octadecenoate isomers (t-18:1) in the trans diet. Compared with the cis diet, the trans diet resulted in the incorporation of approximately 11% t-18:1, an approximately 50% decrease in c-18:1, an approximately 16% decrease in total saturated fatty acids, and an approximately 20% increase in 18:2(n-6) in erythrocyte membrane lipids. The increase in 18:2(n-6) may reflect on homeostatic mechanisms designed to maintain overall membrane fluidity, as no diet-related changes in fluidity were observed with diphenylhexatriene steady state fluorescence polarization. Values observed for insulin binding and insulin receptor number were higher and binding affinity was lower in monkeys fed the cis diet. In the absence of an effect on overall membrane fluidity, altered receptor activity suggests that insulin receptor activity is dynamic, requiring specific fluid membrane subdomains or highly specific fatty acid-protein interactions. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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