| Autor: |
McClellan DA; Department of. Integrative Biology, Brigham Young University, Provo, Utah, USA. david_mcclellan@byu.edu, Palfreyman EJ, Smith MJ, Moss JL, Christensen RG, Sailsbery JK |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular biology and evolution [Mol Biol Evol] 2005 Mar; Vol. 22 (3), pp. 437-55. Date of Electronic Publication: 2004 Oct 27. |
| DOI: |
10.1093/molbev/msi028 |
| Abstrakt: |
Cetaceans have most likely experienced metabolic shifts since evolutionarily diverging from their terrestrial ancestors, shifts that may be reflected in the proteins such as cytochrome b that are responsible for metabolic efficiency. However, accepted statistical methods for detecting molecular adaptation are largely biased against even moderately conservative proteins because the primary criterion involves a comparison of nonsynonymous and synonymous substitution rates (dN/dS); they do not allow for the possibility that adaptation may come in the form of very few amino acid changes. We apply the MM01 model to the possible molecular adaptation of cytochrome b among cetaceans because it does not rely on a dN/dS ratio, instead evaluating positive selection in terms of the amino acid properties that comprise protein phenotypes that selection at the molecular level may act upon. We also apply the codon-degeneracy model (CDM), which focuses on evaluating overall patterns of nucleotide substitution in terms of base exchange, codon position, and synonymy to estimate the overall effect of selection. Using these relatively new models, we characterize the molecular adaptation that has occurred in the cetacean cytochrome b protein by comparing revealed amino acid replacement patterns to those found among artiodactyls, the modern terrestrial mammals found to be most closely related to cetaceans. Our findings suggest that several regions of the cetacean cytochrome b protein have experienced molecular adaptation. Also, these adaptations are spatially associated with domain structure, protein function, and the structure and function of the cytochrome bc(1) complex and its constituents. We also have found a general correlation between the results of the analytical software programs TreeSAAP (which implements the MM01 model) and CDM (which implements the codon-degeneracy model). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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