| Autor: |
Viquez OM; Food Biotechnology Laboratory, Department of Food and Animal Sciences, P.O. Box 1628, Alabama A and M University, Normal AL 35762, USA., Konan KN, Dodo HW |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular immunology [Mol Immunol] 2004 Nov; Vol. 41 (12), pp. 1235-40. |
| DOI: |
10.1016/j.molimm.2004.06.033 |
| Abstrakt: |
Type 1 hypersensitivity to peanut proteins is a well-recognized health problem. Several peanut seed storage proteins have been identified as allergens. Ara h 3, a glycinin protein, is one of the important peanut allergens. Although amino acid and cDNA sequences are available for Ara h 3, there is not information at the genomic level. The objectives of this study were to isolate, sequence, and characterize the genomic clone of peanut allergen, Ara h 3. A peanut genomic library was screened, using two [32P] end-labeled oligonucleotide probes designed based on cDNA sequences of Ara h 3 and Ara h 4. Four positives lambda FIX II clones were obtained after four rounds of screenings. Digestion with Sac I resulted in two fragments of 1.5 and 10 kb hybridizing to the probes. Both fragments were subcloned into p-Bluescript vector and sequenced. The Ara h 3 gene spans 3.5 kb and consists of four exons, three introns, 5' and 3' flanking regions. The open reading frame is 2008 bp long and can encode a polypeptide of 538 amino acids residues. Sequences analogous to a TATA-box (TATAAAT), CAAT-box (AGGA), G-box (TCCTACGTGTCC) and several cis-elements were found in the promoter region. In the 3' downstream region, three polyadenylation signals (AATAAA) were identified. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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