| Autor: |
Collins JE; The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK., Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y, Huckle EJ, Beare DM, Dunham I |
| Jazyk: |
angličtina |
| Zdroj: |
Genome biology [Genome Biol] 2004; Vol. 5 (10), pp. R84. Date of Electronic Publication: 2004 Sep 30. |
| DOI: |
10.1186/gb-2004-5-10-r84 |
| Abstrakt: |
We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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