| Autor: |
Gomulski LM; Department of Animal Biology, University of Pavia, Piazza Botta 9, 127100 Pavia, Italy., Brogna S, Babaratsas A, Gasperi G, Zacharopoulou A, Savakis C, Bourtzis K |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of molecular evolution [J Mol Evol] 2004 Jun; Vol. 58 (6), pp. 732-42. |
| DOI: |
10.1007/s00239-004-2596-9 |
| Abstrakt: |
The first intron of the gene encoding one of the alcohol dehydrogenase isoenzymes (ADH-1) in Ceratitis capitata is highly polymorphic in size. Five size variants of this intron were isolated from different strains and populations and characterized. Restriction map and sequence analysis showed that the intron size polymorphism is due to the presence or absence of (a) a copy of a defective mariner-like element, postdoc; (b) an approximately 550-bp 3' indel which exhibits no similarity to any known sequence; and (c) a central duplication of 704 bp consisting of part of the 3' end of the postdoc element, the region between postdoc and the 3' indel, and the first 20 bp of the 3' indel. The homologous Adh-1 intron was amplified from the congeneric species, Ceratitis rosa, in order to obtain an outgroup for comparative and phylogenetic analyses. The C. rosa introns were polymorphic in size, ranging from about 1100 to 2000 bp, the major difference between them being the presence or absence of a mariner-like element Crmar2, unrelated to the postdoc element. Phylogenetic analysis suggests that the shorter intron variants in C. capitata may represent the ancestral form of the intron, the longest variants apparently being the most recent. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
